Chemiluminescence Analyzer
Chemiluminescence Analyzer Outline
What is Chemiluminescence?
"Chemiluminescence" (chemical luminescence) is the phenomenon arising from chemical reactions. Compounds generated in an excited state by chemical reactions, when released to the ground state, discharge light. Exposed to the atmosphere almost all materials generate chemiluminescence as a result of oxidation reactions. The Chemiluminescence Analyzer (CLA series) is the world’s most sensitive photon counting device. It detect down to 50 photons/cm2/sec (about 1/10000 of a firefly) 10-15 Watts.
Chemiluminescence Intensity
Many things emit ultra-weak light invisible to the human eye in the form of Chemiluminescence, Bioluminescence or Biophoton and research studies are carried out in several field to detect such invisible light. Especially Chemiluminescence is able to estimate the oxidation or deterioration levels of organic samples. Our instrument can be applied not only this oxidation field but also in a variety of other fields.
formed during oxidation
The products of luminescence according to the Russell Mechanism are singlet oxygen and excited carbonyl arising from the bimolecular degradation of hydroperoxides*.
When an excited carbonyl species is released to the ground state, it releases its energy as a photon of light.
Therefore, by following this chemiluminescence, it is possible to measure the degree of oxidation or degradation of a sample. This is a unique and extremely sensitive measure of oxidation.Auto oxidation mechanism
Samples are oxidized by heat, light or other stimulators. Then, according to the oxidation reaction, R・ or ROO・ will be produced and changing to peroxides (ROOH).
When the peroxides are decomposed, peroxyradicals (ROO・) will be produced again and 2 molecules of peroxyradicals will produce the excited carbonyls and active oxygen such as singlet oxygen.
When the excited compounds are released to the ground state, energy is released as heat or light. So, by measuring chemiluminescence, we can estimate the amount of peroxides or the level of a sample’s oxidation.Typical Chemiluminescence(CL)Time-course Change
When the sample placed in the sample chamber is heated, surface oxidized products such as peroxides decompose and produce CL (peak ①).
This CL peak indicates the oxidation level at that time. After that, the CL pattern changes depending on the atmosphere. When measured under N2, CL decreases to 0 (zero). Under O2, the sample is oxidized by heat and O2.
For a limited time anti-oxidants inhibit the oxidation and suppress the CL(②).But after all the anti-oxidants are consumed, the auto-oxidation mechanism commences (③).
This point is the oxidation induction time (OIT) (④). OIT provides an estimate of the oxidation stability of NEW samples.
CL measurement has the merit of being "easy" and "fast" in assessing the oxidation stability of new samples. There is no necessity to oxidize the samples before measurement.
Chemiluminescence Analyzer Series
CLA-FS4
A most highly sensitive instrument using Photomultiplier (PMT) as a detector. CL intensity and CL spectrum can be measured. Our standard product applicable to any study.
CLA-FL2
Using Photomultiplier (PMT) as a detector. This model is used with high performance liquid Chromatograpy (HPLC) and the value output from 0 to 1V. A recorder is needed to process the data.
CLA-ID4
Uses Photomultiplier (PMT) as a detector – a very high sensitive detector measuring CL intensity only.
CLA-IMG
Uses CCD Camera as a detector. allowing a CL image of the oxidation area of in a sample to be taken. A maximum 6 samples can be measured at the same time.
CLA-SP3
Employs a CCD Camera and Diffraction grating. Spectral data with high resolution in a very short time (0.1 sec) can be measured
Sample Chamber
CLS-SH1
Batch Type sample chamber for maximum φ20mm samples.
Operating range: room temp. to 350 degree with facility for varying the heating rate.
CLS-ST3
Batch Type sample chamber for maximum φ50mm samples.
Operating range: room temp. to 160 degree.
CLS-MX3
Batch Type sample chamber for maximum φ50mm samples.
Operating range: room temp. to 100 degree with facility for mixing samples using a stirrer.
CLS-FL
Flow type sample chamber.
Two inlet ports for introducing liquid samples for mixing inside the chamber, and an outlet port for discharging the mixture/product.
CLS-MS
Used with CLA-IMG.
6 samples of maximum φ20mm can be measured.
Operating range room temp. to 160 degree.
CLS-LA
Incorporates a Laser Diode to facilitate sample irradiation (laser power ～ 0.2mW).
